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验证数据展示

  • WB analysis using 83248-4-RR

    WB analysis using 83248-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83248-4-RR (CBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83248-4-RR

    IF Staining of HeLa using 83248-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83248-4-RR

    IF Staining of HeLa using 83248-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83248-4-RR

    FC experiment of HeLa using 83248-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cbx1 Recombinant Antibody (83248-4-RR, Clone:240075H6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83248-4-RR

    Affinity and Kinetic Characterization of 83248-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83248-4-RR against Human CBX1 were performed. The affinity constant is 0.605 nM.

CBX1 Recombinant antibody

CBX1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83248-4-RR

产品说明书

CloneNo. 240075H6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, mouse spleen tissue, mouse brain tissue, mouse kidney tissue, rat brain tissue, human kidney tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CBX1 Ready-To-Use IHC Kit. CBX1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83248-4-RR targets CBX1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 CBX1 fusion protein Ag34901 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromobox homolog 1 (HP1 beta homolog Drosophila )
别名 HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX
计算分子量 27 kDa
观测分子量27 kDa
GenBank蛋白编号BC002609
基因名称 CBX1
Gene ID (NCBI) 10951
RRIDAB_3670923
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP83916
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

实验方案

Product Specific Protocols
WB protocol for CBX1 antibody 83248-4-RRDownload protocol
IF protocol for CBX1 antibody 83248-4-RRDownload protocol
FC protocol for CBX1 antibody 83248-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols