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验证数据展示

  • WB analysis using 83248-4-RR

    WB analysis using 83248-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83248-4-RR (CBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83248-4-RR

    IF Staining of HeLa using 83248-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83248-4-RR

    IF Staining of HeLa using 83248-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83248-4-RR

    FC experiment of HeLa using 83248-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cbx1 Recombinant Antibody (83248-4-RR, Clone:240075H6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83248-4-RR

    Affinity and Kinetic Characterization of 83248-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83248-4-RR against Human CBX1 were performed. The affinity constant is 0.605 nM.

CBX1 Recombinant antibody

CBX1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83248-4-RR

Print datasheet

CloneNo. 240075H6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, mouse spleen tissue, mouse brain tissue, mouse kidney tissue, rat brain tissue, human kidney tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CBX1 Ready-To-Use IHC Kit. CBX1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83248-4-RR targets CBX1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen CBX1 fusion protein Ag34901 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromobox homolog 1 (HP1 beta homolog Drosophila )
Synonyms HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX
Calculated Molecular Weight 27 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC002609
Gene Symbol CBX1
Gene ID (NCBI) 10951
RRIDAB_3670923
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP83916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

实验方案

Product Specific Protocols
WB protocol for CBX1 antibody 83248-4-RRDownload protocol
IF protocol for CBX1 antibody 83248-4-RRDownload protocol
FC protocol for CBX1 antibody 83248-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols