验证数据展示
经过测试的应用
| Positive WB detected in | Various cell, HEK-293 cell, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18544-1-AP targets CBX4 in WB, IF/ICC, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, PLA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | CBX4 fusion protein Ag13425 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromobox homolog 4 (Pc class homolog, Drosophila) |
| 别名 | Pc2, NBP16, hPc2, EC:2.3.2.-, E3 SUMO-protein ligase CBX4 |
| 计算分子量 | 61 kDa |
| 观测分子量 | ~75 kDa |
| GenBank蛋白编号 | BC014967 |
| 基因名称 | CBX4 |
| Gene ID (NCBI) | 8535 |
| RRID | AB_2878552 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00257 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CBX4 antibody 18544-1-AP | Download protocol |
| IF protocol for CBX4 antibody 18544-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes. | ||
Bioengineered Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis.
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