验证数据展示
经过测试的应用
| Positive WB detected in | Various cell, HEK-293 cell, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18544-1-AP targets CBX4 in WB, IF/ICC, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF, PLA |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | CBX4 fusion protein Ag13425 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | chromobox homolog 4 (Pc class homolog, Drosophila) |
| Synonyms | Pc2, NBP16, hPc2, EC:2.3.2.-, E3 SUMO-protein ligase CBX4 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | ~75 kDa |
| GenBank Accession Number | BC014967 |
| Gene Symbol | CBX4 |
| Gene ID (NCBI) | 8535 |
| RRID | AB_2878552 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00257 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CBX4 antibody 18544-1-AP | Download protocol |
| IF protocol for CBX4 antibody 18544-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes. | ||
Bioengineered Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis.
| ||
