验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse kidney tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12239-1-AP targets CBY1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CBY1 fusion protein Ag2882 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chibby homolog 1 (Drosophila) |
| 别名 | arb1, ARPP binding protein, C22orf2, CBY, CBY1, chibby homolog 1 (Drosophila), Cytosolic leucine rich protein, HS508I15A, PGEA1, PIGEA 14, PIGEA14, Protein chibby homolog 1 |
| 计算分子量 | 126 aa, 14 kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | BC016139 |
| 基因名称 | CBY1 |
| Gene ID (NCBI) | 25776 |
| RRID | AB_10951858 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y3M2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CBY1 antibody 12239-1-AP | Download protocol |
| IHC protocol for CBY1 antibody 12239-1-AP | Download protocol |
| IP protocol for CBY1 antibody 12239-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation. | ||
Dev Dyn DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism. | ||
Medicine (Baltimore) Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma | ||
Adv Sci (Weinh) The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers | ||
Heliyon MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling |
