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CC2D2A Polyclonal antibody

CC2D2A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 22293-1-AP

Print datasheet

Synonyms

CC2D2A, JBTS9, KIAA1345, MKS6

验证数据展示

  • IHC staining of human lung cancer using 22293-1-AP

    IHC staining of human lung cancer using 22293-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 22293-1-AP (CC2D2A Antibody) at dilution of 1:50(under 10x lens).

  • IHC staining of human lung cancer using 22293-1-AP

    IHC staining of human lung cancer using 22293-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 22293-1-AP (CC2D2A Antibody) at dilution of 1:50(under 40x lens).

  • IF Staining of hTERT-RPE1 cells using 22293-1-AP

    IF Staining of hTERT-RPE1 cells using 22293-1-AP

    IF result (strong nucleus and the base stain) of anti-CC2D2A (22293-1-AP, 1:50) with serum-starved hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.

  • WB analysis using 22293-1-AP

    WB analysis using 22293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22293-1-AP (CC2D2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22293-1-AP targets CC2D2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil and C2 domain containing 2A
Synonyms CC2D2A, JBTS9, KIAA1345, MKS6
Calculated Molecular Weight 186 kDa
Observed Molecular Weight 190 kDa
GenBank Accession NumberNM_020785
Gene Symbol CC2D2A
Gene ID (NCBI) 57545
RRIDAB_2879063
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2K1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CC2D2A, also named as KIAA1345, may be involved in cilia formation. CC2D2A encodes a protein with similar overall structure to RPGRIP1L, including coiled-coil domains, a C2 domain, and an overlapping centrosomal protein-related domain. CC2D2A physically interacts with CEP290, and loss of Cc2d2a function in the zebrafish sentinel mutant results in abnormal body shape and pronephric (kidney) cysts that is strongly exacerbated by knockdown of Cep290 function. (PMID:19778711 )Defects in CC2D2A are the cause of Meckel syndrome type 6 (MKS6). Defects in CC2D2A are the cause of Joubert syndrome type 9 (JBTS9). The antibody is specific to CC2D2A.

实验方案

Product Specific Protocols
WB protocol for CC2D2A antibody 22293-1-APDownload protocol
IHC protocol for CC2D2A antibody 22293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Dev Cell

Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.

Authors - Quynh P H Nguyen
View Article

Mol Med Rep

Novel CC2D2A compound heterozygous mutations cause Joubert syndrome.

Authors - Daimin Xiao
View Article
humanWB

Ann Hum Genet

Identification of pathogenic deep intronic variant and exonic LINE-1 insertion in a patient with Meckel syndrome

Authors - Sachiko Miyamoto
View Article