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验证数据展示

  • WB analysis using 25232-1-AP

    WB analysis using 25232-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25232-1-AP (CCAR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 25232-1-AP

    IHC staining of human colon using 25232-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCAR1 antibody included
  • IHC staining of human colon using 25232-1-AP

    IHC staining of human colon using 25232-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCAR1 antibody included
  • IF Staining of A431 using 25232-1-AP

    IF Staining of A431 using 25232-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (25232-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • IF Staining of A431 using 25232-1-AP

    IF Staining of A431 using 25232-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (25232-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

CCAR1 Polyclonal antibody

CCAR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25232-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RP11 437A18.1, DIS, Death inducer with SAP domain, CARP-1, CARP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue, mouse colon tissue, rat colon tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CCAR1 Ready-To-Use IHC Kit. CCAR1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25232-1-AP targets CCAR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CCAR1 fusion protein Ag18970 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle and apoptosis regulator 1
Synonyms RP11 437A18.1, DIS, Death inducer with SAP domain, CARP-1, CARP1
Calculated Molecular Weight 1150 aa, 133 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC132725
Gene Symbol CCAR1
Gene ID (NCBI) 55749
RRIDAB_3085778
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IX12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cell cycle and apoptosis regulator 1 (CCAR1) was originally identified from a functional screening and implicated in retinoid-induced cell cycle arrest and apoptosis. It has been shown that, in breast cancer cells, CCAR1 regulates apoptosis induced by various agents, including chemotherapeutics adriamycin, etoposide, and EGFR inhibitor. It is also involved in mediating anchorage-independent growth of colon carcinoma cells. In addition, CCAR1 plays the role as a co-activator of nuclear receptors to recruit mediator complex. It was found to associate with components of the Mediator and p160 coactivator complexes and is recruited to endogenous NR target genes. CCAR1 is also a binding partner of b-catenin and assists b-catenin in transcriptional activation of Wnt target genes.

实验方案

Product Specific Protocols
WB protocol for CCAR1 antibody 25232-1-APDownload protocol
IHC protocol for CCAR1 antibody 25232-1-APDownload protocol
IF protocol for CCAR1 antibody 25232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols