• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83936-4-RR (same clone as 83936-4-PBS)

    WB analysis using 83936-4-RR (same clone as 83936-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83936-4-RR (CCAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83936-4-RR (same clone as 83936-4-PBS)

    IF Staining of A431 using 83936-4-RR (same clone as 83936-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (83936-4-RR, Clone: 240980C12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 83936-4-RR (same clone as 83936-4-PBS)

    IHC staining of human colon using 83936-4-RR (same clone as 83936-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83936-4-RR (CCAR1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCAR1 antibody included
  • IHC staining of human colon using 83936-4-RR (same clone as 83936-4-PBS)

    IHC staining of human colon using 83936-4-RR (same clone as 83936-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83936-4-RR (CCAR1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCAR1 antibody included

CCAR1 Recombinant antibody, PBS Only

CCAR1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83936-4-PBS

产品说明书

CloneNo. 240980C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

240980C12, CARP 1, CARP1, CARP-1, Death inducer with SAP domain

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CCAR1 Ready-To-Use IHC Kit. CCAR1 primary antibody included.

产品信息

83936-4-PBS targets CCAR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CCAR1 fusion protein Ag18786 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cell division cycle and apoptosis regulator 1
别名 240980C12, CARP 1, CARP1, CARP-1, Death inducer with SAP domain
计算分子量 1150 aa, 133 kDa
观测分子量150 kDa
GenBank蛋白编号BC132725
基因名称 CCAR1
Gene ID (NCBI) 55749
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8IX12
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cell cycle and apoptosis regulator 1 (CCAR1) was originally identified from a functional screening and implicated in retinoid-induced cell cycle arrest and apoptosis. It has been shown that, in breast cancer cells, CCAR1 regulates apoptosis induced by various agents, including chemotherapeutics adriamycin, etoposide, and EGFR inhibitor. It is also involved in mediating anchorage-independent growth of colon carcinoma cells. In addition, CCAR1 plays the role as a co-activator of nuclear receptors to recruit mediator complex. It was found to associate with components of the Mediator and p160 coactivator complexes and is recruited to endogenous NR target genes. CCAR1 is also a binding partner of b-catenin and assists b-catenin in transcriptional activation of Wnt target genes.