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  • Featured Product
  • KD/KO Validated

CCAR2 Monoclonal antibody, PBS Only

CCAR2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D10F8

Cat no : 66497-1-PBS

Print datasheet

Synonyms

cell cycle and apoptosis regulator 2, DBC1, DBC-1, KIAA1967, DBC.1

验证数据展示

  • WB analysis using 66497-1-Ig (same clone as 66497-1-PBS)

    WB analysis using 66497-1-Ig (same clone as 66497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66497-1-Ig (same clone as 66497-1-PBS)

    WB analysis of HEK-293 using 66497-1-Ig (same clone as 66497-1-PBS)

    WB result of CCAR2 antibody (66497-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCAR2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

    IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66497-1-Ig (CCAR2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 66497-1-Ig (same clone as 66497-1-PBS)

    FC experiment of U2OS using 66497-1-Ig (same clone as 66497-1-PBS)

    1X10^6 U2OS cells were intracellularly stained with 0.2 ug Anti-Human CCAR2 (66497-1-Ig, Clone:2D10F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

    IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCAR2 antibody (66497-1-Ig, Clone: 2D10F8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DBC1/CCAR2 Ready-To-Use IHC Kit. DBC1/CCAR2 primary antibody included.

产品信息

66497-1-PBS targets CCAR2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen CCAR2 fusion protein Ag18374 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name KIAA1967
Synonyms cell cycle and apoptosis regulator 2, DBC1, DBC-1, KIAA1967, DBC.1
Calculated Molecular Weight 923 aa, 103 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC018269
Gene Symbol CCAR2
Gene ID (NCBI) 57805
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N163
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).