验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, HeLa cells, L02 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 10 publications below |
| IHC | See 1 publications below |
产品信息
12156-1-AP targets CCBL1/KAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CCBL1/KAT1 fusion protein Ag2828 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cysteine conjugate-beta lyase, cytoplasmic |
| 别名 | CCBL1, CCBL1/KAT1, Glutamine transaminase K, GTK, KAT1, KATI, KYAT1, Kynurenine aminotransferase I |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC013069 |
| 基因名称 | CCBL1 |
| Gene ID (NCBI) | 883 |
| RRID | AB_10666849 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16773 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCBL1 encodes kynurenine aminotransferase 1 (KAT1), a key enzyme involved in kynurenine pathway. CCBL1 catalyzes the production of kynurenic acid, a powerful endogenous excitatory amino acid receptor antagonist that is widely regarded as the potent neuroprotective agent.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCBL1/KAT1 antibody 12156-1-AP | Download protocol |
| IF protocol for CCBL1/KAT1 antibody 12156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience to stress-induced depression. | ||
J Intern Med Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans. | ||
Brain Behav Immun Kynurenine pathway is altered in BDNF Val66Met knock-in mice: Effect of physical exercise. | ||
Am J Physiol Cell Physiol Endurance exercise increases skeletal muscle kynurenine aminotransferases and plasma kynurenic acid in humans. | ||
Sci Rep Caffeine protects against stress-induced murine depression through activation of PPARγC1α-mediated restoration of the kynurenine pathway in the skeletal muscle. |
