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验证数据展示

  • WB analysis using 30296-1-AP

    WB analysis using 30296-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30296-1-AP (CCBL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 30296-1-AP

    IF Staining of HeLa using 30296-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CCBL1 antibody (30296-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CCBL1 Polyclonal antibody

CCBL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

CCBL1, FLJ95217, Glutamine transaminase K, GTK, KAT1, KATI, Kynurenine aminotransferase I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, U-87 MG cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30296-1-AP targets CCBL1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 CCBL1 fusion protein Ag32316 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cysteine conjugate-beta lyase, cytoplasmic
别名 CCBL1, FLJ95217, Glutamine transaminase K, GTK, KAT1, KATI, Kynurenine aminotransferase I
观测分子量 48 kDa
GenBank蛋白编号BC033685
基因名称 CCBL1
Gene ID (NCBI) 883
RRIDAB_2935538
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16773
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCBL1 encodes kynurenine aminotransferase 1 (KAT1), a key enzyme involved in kynurenine pathway. CCBL1 catalyzes the production of kynurenic acid, a powerful endogenous excitatory amino acid receptor antagonist that is widely regarded as the potent neuroprotective agent.

实验方案

Product Specific Protocols
WB protocol for CCBL1 antibody 30296-1-APDownload protocol
IF protocol for CCBL1 antibody 30296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols