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验证数据展示

  • IHC staining of human tonsillitis using 60046-1-Ig

    IHC staining of human tonsillitis using 60046-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60046-1-Ig (ACKR2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 60046-1-Ig

    IHC staining of human tonsillitis using 60046-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60046-1-Ig (ACKR2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 60046-1-Ig

    IF Staining of human tonsillitis using 60046-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ACKR2 antibody (60046-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 60046-1-Ig

    IF Staining of human tonsillitis using 60046-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ACKR2 antibody (60046-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ACKR2 Monoclonal antibody

ACKR2 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 60046-1-Ig

产品说明书

CloneNo. 1A3A2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, IF-P, ELISA

别名

ACKR2, C C chemokine receptor D6, CCBP2, CCR10, CCR9, chemokine binding protein 2, Chemokine binding protein D6, Chemokine receptor CCR 10, Chemokine receptor CCR 9, CMKBR9, D6, hD6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60046-1-Ig targets ACKR2 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 ACKR2 fusion protein Ag1941 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chemokine binding protein 2
别名 ACKR2, C C chemokine receptor D6, CCBP2, CCR10, CCR9, chemokine binding protein 2, Chemokine binding protein D6, Chemokine receptor CCR 10, Chemokine receptor CCR 9, CMKBR9, D6, hD6
计算分子量 43 kDa
GenBank蛋白编号BC008816
基因名称 ACKR2
Gene ID (NCBI) 1238
RRIDAB_2071883
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00590
储存缓冲液 PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACKR2, also named as CCBP2 or chemokine receptor D6, is a heptahelical membrane protein expressed by lymphatic endothelial cells, binding with high affinity to multiple proinflammatory CC chemokines.

实验方案

Product Specific Protocols
IHC protocol for ACKR2 antibody 60046-1-IgDownload protocol
IF protocol for ACKR2 antibody 60046-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols