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ACKR2 Monoclonal antibody

ACKR2 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A3A2

Cat no : 60046-1-Ig

Print datasheet

Synonyms

ACKR2, C C chemokine receptor D6, CCBP2, CCR10, CCR9, chemokine binding protein 2, Chemokine binding protein D6, Chemokine receptor CCR 10, Chemokine receptor CCR 9, CMKBR9, D6, hD6

验证数据展示

  • IHC staining of human tonsillitis using 60046-1-Ig

    IHC staining of human tonsillitis using 60046-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60046-1-Ig (ACKR2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 60046-1-Ig

    IHC staining of human tonsillitis using 60046-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60046-1-Ig (ACKR2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 60046-1-Ig

    IF Staining of human tonsillitis using 60046-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ACKR2 antibody (60046-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 60046-1-Ig

    IF Staining of human tonsillitis using 60046-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ACKR2 antibody (60046-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60046-1-Ig targets ACKR2 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen ACKR2 fusion protein Ag1941 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chemokine binding protein 2
Synonyms ACKR2, C C chemokine receptor D6, CCBP2, CCR10, CCR9, chemokine binding protein 2, Chemokine binding protein D6, Chemokine receptor CCR 10, Chemokine receptor CCR 9, CMKBR9, D6, hD6
Calculated Molecular Weight 43 kDa
GenBank Accession NumberBC008816
Gene Symbol ACKR2
Gene ID (NCBI) 1238
RRIDAB_2071883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00590
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACKR2, also named as CCBP2 or chemokine receptor D6, is a heptahelical membrane protein expressed by lymphatic endothelial cells, binding with high affinity to multiple proinflammatory CC chemokines.

实验方案

Product Specific Protocols
IHC protocol for ACKR2 antibody 60046-1-IgDownload protocol
IF protocol for ACKR2 antibody 60046-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols