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  • Featured Product
  • KD/KO Validated

SGF29 Polyclonal antibody

SGF29 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24061-1-AP

Print datasheet

Synonyms

CCDC101, SGF29

验证数据展示

  • WB analysis of HeLa using 24061-1-AP

    WB analysis of HeLa using 24061-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24061-1-AP (SGF29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 24061-1-AP

    IHC staining of human liver cancer using 24061-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 10x lens).

  • IHC staining of human liver cancer using 24061-1-AP

    IHC staining of human liver cancer using 24061-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, MDA-MB-453s cells
Positive IHC detected inhuman liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24061-1-AP targets SGF29 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SGF29 fusion protein Ag21261 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 101
Synonyms CCDC101, SGF29
Calculated Molecular Weight 293 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC011981
Gene Symbol SGF29
Gene ID (NCBI) 112869
RRIDAB_2879420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96ES7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAGA-associated factor 29 (SGF29) is a chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes. SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PMID:19103755, PMID:26421618, PMID:21685874, PMID:26578293). Involved in the response to the endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed:23894581). It has a calculated molecular weight of 33 kDa.

实验方案

Product Specific Protocols
WB protocol for SGF29 antibody 24061-1-APDownload protocol
IHC protocol for SGF29 antibody 24061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Authors - Lingyun Long
  • KO Validated
View Article