验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, rat brain tissue, HepG2 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26312-1-AP targets MCU/CCDC109A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | MCU/CCDC109A fusion protein Ag24733 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 109A |
| 别名 | C10orf42, Calcium uniporter protein, CCDC109A, MCU, MCU/CCDC109A |
| 计算分子量 | 35 kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | BC034235 |
| 基因名称 | CCDC109A |
| Gene ID (NCBI) | 90550 |
| RRID | AB_2880474 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NE86 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MCU, also known as CCDC109A, is highly conserved across all eukaryotes. The MCU protein is composed of two coiled-coil domains, two TMDs, and a short motif of amino acids between the two TMDs. Knockdown of MCU dramatically reduces mitochondrial Ca2+ uptake in isolated mitochondria, in permeabilized cells and living cells. MCU has 3 isoforms with MW 40,37 and 35 kDa (refer to UniProt). The observed MW of MCU is mainly between 30 to 35 kDa in paper (PMID: 26341627; 28337252).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCU/CCDC109A antibody 26312-1-AP | Download protocol |
| IHC protocol for MCU/CCDC109A antibody 26312-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Differ Ca2+-mediated mitochondrial inner membrane permeabilization induces cell death independently of Bax and Bak. | ||
Environ Pollut TDCPP and TiO2 NPs aggregates synergistically induce SH-SY5Y cell neurotoxicity by excessive mitochondrial fission and mitophagy inhibition | ||
Cell Rep A mitochondrial inside-out iron-calcium signal reveals drug targets for Parkinson's disease | ||
Int J Mol Sci Effects of MICU1-Mediated Mitochondrial Calcium Uptake on Energy Metabolism and Quality of Vitrified-Thawed Mouse Metaphase II Oocytes | ||
J Agric Food Chem Puerarin Ameliorated PCOS through Preventing Mitochondrial Dysfunction Dependent on the Maintenance of Intracellular Calcium Homeostasis | ||
iScience Matrix stiffness aggravates osteoarthritis progression through H3K27me3 demethylation induced by mitochondrial damage |
