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CCDC115 Polyclonal antibody

CCDC115 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20636-1-AP

Print datasheet

Synonyms

Coiled-coil domain-containing protein 115, ccp1

验证数据展示

  • WB analysis using 20636-1-AP

    WB analysis using 20636-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20636-1-AP (CCDC115 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 20636-1-AP

    WB analysis of mouse brain using 20636-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20636-1-AP (CCDC115 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 20636-1-AP

    IHC staining of human ovary cancer using 20636-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20636-1-AP (CCDC115 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 20636-1-AP

    IHC staining of human ovary cancer using 20636-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20636-1-AP (CCDC115 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, mouse brain tissue, MOLT-4 cells, Y79 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20636-1-AP targets CCDC115 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CCDC115 fusion protein Ag14677 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 115
Synonyms Coiled-coil domain-containing protein 115, ccp1
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC006429
Gene Symbol CCDC115
Gene ID (NCBI) 84317
RRIDAB_10733755
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NT0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC115 antibody 20636-1-APDownload protocol
IHC protocol for CCDC115 antibody 20636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Heliyon

Identification of CCDC115 as an adverse prognostic biomarker in liver cancer based on bioinformatics and experimental analyses

Authors - Chang Su
View Article