验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human placenta tissue, mouse liver tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
27106-1-AP targets CCDC124 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | CCDC124 fusion protein Ag25930 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 124 |
| 别名 | CCDC124 |
| 观测分子量 | 30-32 kDa |
| GenBank蛋白编号 | BC013949 |
| 基因名称 | CCDC124 |
| Gene ID (NCBI) | 115098 |
| RRID | AB_2880758 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96CT7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCDC124 is a novel centrosomal and midbody protein that is highly conserved among species. It is ubiquitously expressed with high expression in brain, placenta, liver, spleen, and prostate. At telophase, CCDC124 dissociates from centrosomes and relocalizes to the midzone, subsequently accumulating at the midbody at cytokinesis. It interacts with the Ras-guanine nucleotide exchange factor 1B (RasGEF1B) and is involved in cytokinesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC124 antibody 27106-1-AP | Download protocol |
| IF protocol for CCDC124 antibody 27106-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
