验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells, CHO cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26500-1-AP targets CCDC134 in WB, ELISA applications and shows reactivity with human, hamster samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, hamster |
| 免疫原 | CCDC134 fusion protein Ag21990 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 134 |
| 别名 | CCDC134 |
| 计算分子量 | 229 aa, 27 kDa |
| 观测分子量 | 27 kda |
| GenBank蛋白编号 | BC017693 |
| 基因名称 | CCDC134 |
| Gene ID (NCBI) | 79879 |
| RRID | AB_3669541 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H6E4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCDC134 (coiled-coil domain containing 134) encodes a secretory protein that can inhibit the MAPK pathway as a novel human MAPK-regulating protein (PMID: 18087676). CCDC134 is in extracellular secreted form, promotes proliferation and activation of CD8(+) T cells, suggesting a cytokine-like function (PubMed: 25125657). CCDC134 is widely expressed in normal adult tissues, tumor tissues and cell lines with the molecular weight of 27 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC134 antibody 26500-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |