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验证数据展示

  • WB analysis using 84106-3-RR

    WB analysis using 84106-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84106-3-RR (CCDC134 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 84106-3-RR

    WB analysis of HEK-293 using 84106-3-RR

    WB result of CCDC134 antibody (84106-3-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC134 transfected HEK-293 cells.

  • FC experiment of U2OS using 84106-3-RR

    FC experiment of U2OS using 84106-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug CCDC134 Recombinant antibody (84106-3-RR, Clone:241212F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84106-3-RR

    Affinity and Kinetic Characterization of 84106-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84106-3-RR against Human CCDC134 were performed. The affinity constant is 1.69 nM.

  • Featured Product
  • KD/KO验证

CCDC134 Recombinant antibody

CCDC134 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84106-3-RR

产品说明书

CloneNo. 241212F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

241212F11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, HL-60 cells, U-251 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84106-3-RR targets CCDC134 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 CCDC134 fusion protein Ag21990 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coiled-coil domain containing 134
别名 241212F11
计算分子量 229 aa, 27 kDa
观测分子量25-27 kDa
GenBank蛋白编号BC017693
基因名称 CCDC134
Gene ID (NCBI) 79879
RRIDAB_3671672
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H6E4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC134 antibody 84106-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols