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CCDC158 Polyclonal antibody

CCDC158 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20396-1-AP

Print datasheet

Synonyms

CCDC158

验证数据展示

  • WB analysis of mouse testis using 20396-1-AP

    WB analysis of mouse testis using 20396-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20396-1-AP (CCDC158 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20396-1-AP

    IHC staining of human kidney using 20396-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 20396-1-AP

    IHC staining of human kidney using 20396-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse testis using 20396-1-AP

    IP experiment of mouse testis using 20396-1-AP

    IP result of anti-CCDC158 (IP:20396-1-AP, 4ug; Detection:20396-1-AP 1:500) with mouse testis tissue lysate 6000ug.

经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20396-1-AP targets CCDC158 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen CCDC158 fusion protein Ag14049 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 158
Synonyms CCDC158
Calculated Molecular Weight 1113 aa, 127 kDa
Observed Molecular Weight 127-140 kDa
GenBank Accession NumberBC035224
Gene Symbol CCDC158
Gene ID (NCBI) 339965
RRIDAB_10694278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5M9N0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC158 antibody 20396-1-APDownload protocol
IHC protocol for CCDC158 antibody 20396-1-APDownload protocol
IP protocol for CCDC158 antibody 20396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

J Cell Physiol

CCDC158: A novel regulator in renal proximal tubular endocytosis unveiled through exome sequencing and interactome analysis

Authors - Tjessa Bondue
View Article