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验证数据展示

  • WB analysis using 31922-1-AP

    WB analysis using 31922-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31922-1-AP (CCDC167 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 31922-1-AP

    IF Staining of U2OS using 31922-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCDC167 antibody (31922-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CCDC167 Polyclonal antibody

CCDC167 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31922-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Transmembrane And Coiled-Coil Domain-Containing Protein C6orf129, HSPC265, Coiled-coil domain-containing protein 167, Coiled-Coil Domain Containing 167, Chromosome 6 Open Reading Frame 129

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HuH-7 cells, LNCaP cells, Raji cells, Ramos cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31922-1-AP targets CCDC167 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CCDC167 fusion protein Ag37080 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 6 open reading frame 129
别名 Transmembrane And Coiled-Coil Domain-Containing Protein C6orf129, HSPC265, Coiled-coil domain-containing protein 167, Coiled-Coil Domain Containing 167, Chromosome 6 Open Reading Frame 129
计算分子量11 kDa
观测分子量11 kDa
GenBank蛋白编号BC108655
基因名称 C6orf129
Gene ID (NCBI) 154467
RRIDAB_3670146
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9P0B6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coiled-coil domain (CCD) constituents are alpha-helix motifs expressed in different types of proteins, which can function in various biological processes, including cell proliferation, migration, and signal transduction (PMID: 9171830; 17428801). The coiled-coil domain containing 167 (CCDC167, also known as HSPC265 and C6orf129) is a membrane protein highly expressed in the lymph node. It is associated with immune function, which could be a therapeutic target for breast cancer and asthma (PMID: 33461170; 31821602).

实验方案

Product Specific Protocols
WB protocol for CCDC167 antibody 31922-1-APDownload protocol
IF protocol for CCDC167 antibody 31922-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols