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CCDC22 Recombinant antibody

CCDC22 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241146A5

Cat no : 83992-1-RR

Print datasheet

Synonyms

JM1, CXorf37, Coiled-coil domain-containing protein 22, Coiled coil domain containing protein 22, coiled coil domain containing 22

验证数据展示

  • WB analysis using 83992-1-RR

    WB analysis using 83992-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83992-1-RR (CCDC22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83992-1-RR

    Affinity and Kinetic Characterization of 83992-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83992-1-RR against Human CCDC22 were performed. The affinity constant is 2.71 nM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83992-1-RR is CCDC22 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CCDC22 fusion protein Ag9980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coiled-coil domain containing 22
Synonyms JM1, CXorf37, Coiled-coil domain-containing protein 22, Coiled coil domain containing protein 22, coiled coil domain containing 22
Calculated Molecular Weight 71 kDa
Observed Molecular Weight71 kDa
GenBank Accession NumberBC000972
Gene Symbol CCDC22
Gene ID (NCBI) 28952
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60826
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC22 is a ubiquitously expressed coiled-coil domain protein. CCDC22 functions in the regulation of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) by interacting with COMMD (copper metabolism Murr1 domain-containing) proteins. ]

实验方案

Product Specific Protocols
WB protocol for CCDC22 antibody 83992-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols