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CCDC28A Polyclonal antibody

CCDC28A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14807-1-AP

Print datasheet

Synonyms

Coiled-coil domain-containing protein 28A, CCRL1AP, CC28A, C6orf80

验证数据展示

  • WB analysis using 14807-1-AP

    WB analysis using 14807-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 14807-1-AP

    IF Staining of MCF-7 using 14807-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CCDC28A antibody (14807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

经过测试的应用

Positive WB detected inA375 cells, HeLa cells, mouse thymus tissue, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14807-1-AP targets CCDC28A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CCDC28A fusion protein Ag6529 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 28A
Synonyms Coiled-coil domain-containing protein 28A, CCRL1AP, CC28A, C6orf80
Calculated Molecular Weight 30 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC000758
Gene Symbol CCDC28A
Gene ID (NCBI) 25901
RRIDAB_2072069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWP9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC28A antibody 14807-1-APDownload protocol
IF protocol for CCDC28A antibody 14807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols