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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 67755-1-Ig

    WB analysis using 67755-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67755-1-Ig (CCDC45 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 67755-1-Ig

    IF Staining of U2OS using 67755-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCDC45 antibody (67755-1-Ig, Clone: 1A12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CCDC45 Monoclonal antibody

CCDC45 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67755-1-Ig

Print datasheet

CloneNo. 1A12A7

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

CCDC45, DKFZp667E1824

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMOLT-4 cells, Jurkat cells, K-562 cells, HL-60 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67755-1-Ig targets CCDC45 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen CCDC45 fusion protein Ag27836 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coiled-coil domain containing 45
Synonyms CCDC45, DKFZp667E1824
Calculated Molecular Weight 821 aa, 95 kDa
Observed Molecular Weight95-100 kDa
GenBank Accession NumberBC009518
Gene Symbol CCDC45
Gene ID (NCBI) 90799
RRIDAB_2918523
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96GE4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC45 antibody 67755-1-IgDownload protocol
IF protocol for CCDC45 antibody 67755-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols