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验证数据展示

  • WB analysis of HeLa using 24416-1-AP

    WB analysis of HeLa using 24416-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24416-1-AP (CWC25 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 24416-1-AP

    IHC staining of human kidney using 24416-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 24416-1-AP

    IHC staining of human kidney using 24416-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 24416-1-AP

    IHC staining of human cervical cancer using 24416-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 24416-1-AP

    IHC staining of human cervical cancer using 24416-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 24416-1-AP

    IF Staining of HepG2 using 24416-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CWC25 antibody (24416-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

CWC25 Polyclonal antibody

CWC25 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24416-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CCDC49, Spliceosome-associated protein homolog CWC25, Pre-mRNA-splicing factor CWC25 homolog, Coiled-coil domain-containing protein 49

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24416-1-AP targets CWC25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CWC25 fusion protein Ag17037 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 49
别名 CCDC49, Spliceosome-associated protein homolog CWC25, Pre-mRNA-splicing factor CWC25 homolog, Coiled-coil domain-containing protein 49
计算分子量 425 aa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC008833
基因名称 CCDC49
Gene ID (NCBI) 54883
RRIDAB_2879535
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXE8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CWC25 antibody 24416-1-APDownload protocol
IHC protocol for CWC25 antibody 24416-1-APDownload protocol
IF protocol for CWC25 antibody 24416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols