验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, L02 cells, mouse lung tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human kidney tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21082-1-AP targets CCDC50 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CCDC50 fusion protein Ag15293 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 50 |
| 别名 | C3orf6, CCDC50, DFNA44, Protein Ymer, YMER |
| 计算分子量 | 482 aa, 56 kDa |
| 观测分子量 | 36-38 kDa |
| GenBank蛋白编号 | BC065004 |
| 基因名称 | CCDC50 |
| Gene ID (NCBI) | 152137 |
| RRID | AB_10695770 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IVM0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC50 antibody 21082-1-AP | Download protocol |
| IHC protocol for CCDC50 antibody 21082-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep CCDC50 promotes tumor growth through regulation of lysosome homeostasis
| ||
EMBO Rep CCDC50 suppresses NLRP3 inflammasome activity by mediating autophagic degradation of NLRP3
| ||
Autophagy CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity |
