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  • Featured Product
  • KD/KO Validated

CCDC51 Polyclonal antibody

CCDC51 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20465-1-AP

Print datasheet

Synonyms

MITOK, Mitochondrial potassium channel, Coiled-coil domain-containing protein 51

验证数据展示

  • WB analysis using 20465-1-AP

    WB analysis using 20465-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20465-1-AP (CCDC51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 20465-1-AP

    IHC staining of human ovary tumor using 20465-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20465-1-AP (CCDC51 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 20465-1-AP

    IF Staining of HeLa using 20465-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20465-1-AP (CCDC51 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20465-1-AP targets CCDC51 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CCDC51 fusion protein Ag14301 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 51
Synonyms MITOK, Mitochondrial potassium channel, Coiled-coil domain-containing protein 51
Calculated Molecular Weight 411 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC004341
Gene Symbol CCDC51
Gene ID (NCBI) 79714
RRIDAB_10700001
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96ER9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC51 antibody 20465-1-APDownload protocol
IHC protocol for CCDC51 antibody 20465-1-APDownload protocol
IF protocol for CCDC51 antibody 20465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF,WB

bioRxiv

Human CCDC51 and yeast Mdm33 are functionally conserved mitochondrial inner membrane proteins that demarcate a subset of organelle fission events

Authors - Alia R Edington
  • KD Validated
View Article