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CCDC53 Polyclonal antibody

CCDC53 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24445-1-AP

Print datasheet

Synonyms

CCDC53, CGI 116, WASH complex subunit CCDC53, WASHC3, x0009

验证数据展示

  • IHC staining of human placenta using 24445-1-AP

    IHC staining of human placenta using 24445-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24445-1-AP (CCDC53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 24445-1-AP

    IHC staining of human placenta using 24445-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24445-1-AP (CCDC53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman placenta tissue, human breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

24445-1-AP targets CCDC53 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CCDC53 fusion protein Ag19831 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 53
Synonyms CCDC53, CGI 116, WASH complex subunit CCDC53, WASHC3, x0009
Calculated Molecular Weight 194 aa, 21 kDa
GenBank Accession NumberBC010889
Gene Symbol CCDC53
Gene ID (NCBI) 51019
RRIDAB_2879551
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3C0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CCDC53 antibody 24445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Architecture of the human interactome defines protein communities and disease networks.

Authors - Edward L Huttlin
View Article
humanWB

Nat Commun

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Authors - Yunkai Zhu
View Article