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CCDC56/COA3 Polyclonal antibody

CCDC56/COA3 Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 20427-1-AP

Print datasheet

Synonyms

CCDC56, COA3, HSPC009

验证数据展示

  • IHC staining of human colon cancer using 20427-1-AP

    IHC staining of human colon cancer using 20427-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 20427-1-AP

    IHC staining of human colon cancer using 20427-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 20427-1-AP

    IF Staining of U2OS using 20427-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCDC56 antibody (20427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of A431 using 20427-1-AP

    IF Staining of A431 using 20427-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC56 antibody (20427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, U2OS cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

20427-1-AP targets CCDC56/COA3 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CCDC56/COA3 fusion protein Ag14260 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 56
Synonyms CCDC56, COA3, HSPC009
Calculated Molecular Weight 106 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC002698
Gene Symbol CCDC56
Gene ID (NCBI) 28958
RRIDAB_10695182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2R0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CCDC56/COA3 antibody 20427-1-APDownload protocol
IF protocol for CCDC56/COA3 antibody 20427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Am J Cancer Res

COA3 overexpression promotes non-small cell lung cancer metastasis by reprogramming glucose metabolism

Authors - Hongwei Lin
View Article