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CCDC59 Polyclonal antibody

CCDC59 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26387-1-AP

Print datasheet

Synonyms

BR22, CCDC59, HSPC128, TAP26, TTF 1 associated protein 26, TTF 1 associated protein BR2

验证数据展示

  • WB analysis of MCF-7 using 26387-1-AP

    WB analysis of MCF-7 using 26387-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26387-1-AP (CCDC59 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 26387-1-AP

    IP experiment of HepG2 using 26387-1-AP

    IP result of anti-CCDC59 (IP:26387-1-AP, 4ug; Detection:26387-1-AP 1:1000) with HepG2 cells lysate 4000ug.

  • IHC staining of human lung cancer using 26387-1-AP

    IHC staining of human lung cancer using 26387-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26387-1-AP (CCDC59 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 26387-1-AP

    IHC staining of human lung cancer using 26387-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26387-1-AP (CCDC59 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26387-1-AP is CCDC59 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen CCDC59 fusion protein Ag23852 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 59
Synonyms BR22, CCDC59, HSPC128, TAP26, TTF 1 associated protein 26, TTF 1 associated protein BR2
Observed Molecular Weight 34-38 kDa
GenBank Accession NumberBC020647
Gene Symbol CCDC59
Gene ID (NCBI) 29080
RRIDAB_2880499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC59 antibody 26387-1-APDownload protocol
IHC protocol for CCDC59 antibody 26387-1-APDownload protocol
IP protocol for CCDC59 antibody 26387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols