验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, A549 cells, U2OS cells, LNCaP cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67637-1-Ig targets CCDC6 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | CCDC6 fusion protein Ag6952 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | coiled-coil domain containing 6 |
Synonyms | CCDC6, D10S170, FLJ32286, H4, Protein H4, PTC, TPC, TST1 |
Calculated Molecular Weight | 474 aa, 53 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC036757 |
Gene Symbol | CCDC6 |
Gene ID (NCBI) | 8030 |
RRID | AB_2882838 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q16204 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCDC6 (Coiled-coil domain-containing protein 6) is also named as Protein H4, D10S170 and TST1. CCDC6, was initially isolated as part of a tumorigenic DNA originated by the fusion of CCDC6 with the tyrosine kinase of RET receptor. CCDC6 has been considered as an accidental partner of the RET protooncogene, providing the promoter and the first 101 aa necessary for the constitutive activation of the oncogenic Tyrosine Kinase (TK) RET in thyroid cells. The 65 kDa product of CCDC6 has a nuclear transfer sequence with no transmembrane domains and is predicted to locate in both the nucleus and the cytoplasm (PMID: 29044514). The CCDC6 is a phosphoprotein, predicted target of several S/T kinases which can modulate the protein stability and the intracellular shuttling into the nucleus upon different cellular signals mediated by ERK1/2, ATM and CDK1/2. CCDC6 is involved in cellular response to DNA damage mediated by ATM, with the final result of promoting cellular apoptosis. CCDC6 depleted cells are considered defective of DNA repair checkpoint and proceed faster than the control cells in the cell cycle upon induced DNA damage (PMID: 22655027).
实验方案
Product Specific Protocols | |
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WB protocol for CCDC6 antibody 67637-1-Ig | Download protocol |
IF protocol for CCDC6 antibody 67637-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |