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CCDC65 Polyclonal antibody

CCDC65 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 24376-1-AP

Print datasheet

Synonyms

Testis development protein NYD-SP28, NYD SP28, Dynein regulatory complex subunit 2, DRC2, Coiled-coil domain-containing protein 65

验证数据展示

  • WB analysis of mouse testis using 24376-1-AP

    WB analysis of mouse testis using 24376-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24376-1-AP (CCDC65 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 24376-1-AP

    IP experiment of mouse testis using 24376-1-AP

    IP result of anti-CCDC65 (IP:24376-1-AP, 4ug; Detection:24376-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24376-1-AP targets CCDC65 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CCDC65 fusion protein Ag19357 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 65
Synonyms Testis development protein NYD-SP28, NYD SP28, Dynein regulatory complex subunit 2, DRC2, Coiled-coil domain-containing protein 65
Calculated Molecular Weight 484 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC039317
Gene Symbol CCDC65
Gene ID (NCBI) 85478
RRIDAB_2879513
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXS2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC65, also known as DRC2, is a sperm tail protein highly expressed in adult testis, spermatocytes, and spermatids. It is a component of the nexin-dynein regulatory complex (N-DRC), a structure important for regulating and orchestrating dynein activity. Mutations in CCDC65 are associated with primary ciliary dyskinesia (PCD), a genetic disorder that affects the structure and function of cilia(PMID: 23991085).

实验方案

Product Specific Protocols
WB protocol for CCDC65 antibody 24376-1-APDownload protocol
IP protocol for CCDC65 antibody 24376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Sci

CCDC65, a Gene Knockout that leads to Early Death of Mice, acts as a potentially Novel Tumor Suppressor in Lung Adenocarcinoma.

Authors - Ziyan Zhang
View Article