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CCDC71 Polyclonal antibody

CCDC71 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28152-1-AP

Print datasheet

Synonyms

CCDC71, FLJ12800, FLJ13629

验证数据展示

  • WB analysis using 28152-1-AP

    WB analysis using 28152-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28152-1-AP (CCDC71 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28152-1-AP

    IHC staining of human breast cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 28152-1-AP

    IHC staining of human breast cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28152-1-AP targets CCDC71 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen CCDC71 fusion protein Ag27446 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 71
Synonyms CCDC71, FLJ12800, FLJ13629
Calculated Molecular Weight 467 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC035516
Gene Symbol CCDC71
Gene ID (NCBI) 64925
RRIDAB_2881075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IV32
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC71 antibody 28152-1-APDownload protocol
IHC protocol for CCDC71 antibody 28152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols