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CCDC72 Polyclonal antibody

CCDC72 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20393-1-AP

Print datasheet

Synonyms

Translation machinery-associated protein 7, TMA7, HSPC330, HSPC016, Coiled-coil domain-containing protein 72

验证数据展示

  • IP experiment of A549 using 20393-1-AP

    IP experiment of A549 using 20393-1-AP

    IP result of anti-CCDC72 (IP:20393-1-AP, 4ug; Detection:20393-1-AP 1:500) with A549 cells lysate 2400 ug.

  • WB analysis of A549 using 20393-1-AP

    WB analysis of A549 using 20393-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 20393-1-AP (CCDC72 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 20393-1-AP

    IHC staining of human skin using 20393-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 20393-1-AP

    IHC staining of human skin using 20393-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A549 using 20393-1-AP

    IF Staining of A549 using 20393-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, MCF-7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20393-1-AP targets CCDC72 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CCDC72 fusion protein Ag13792 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 72
Synonyms Translation machinery-associated protein 7, TMA7, HSPC330, HSPC016, Coiled-coil domain-containing protein 72
Calculated Molecular Weight 64 kDa, 7 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC001102
Gene Symbol CCDC72
Gene ID (NCBI) 51372
RRIDAB_10667404
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2S6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC72, also named as TMA7, is a novel modulator for hair follicle development. CCDC72 has the promoter activity and regulation of gene expression.

实验方案

Product Specific Protocols
WB protocol for CCDC72 antibody 20393-1-APDownload protocol
IHC protocol for CCDC72 antibody 20393-1-APDownload protocol
IF protocol for CCDC72 antibody 20393-1-APDownload protocol
IP protocol for CCDC72 antibody 20393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Int J Biol Sci

IGF2BP3 Regulates TMA7-mediated Autophagy and Cisplatin Resistance in Laryngeal Cancer via m6A RNA Methylation

Authors - Like Yang
View Article
mouseWB

bioRxiv

Genetic deficiency of ribosomal rescue factor HBS1L causes retinal dystrophy associated with Pelota and EDF1 depletion

Authors - Shiyu Luo
View Article
mouseWB

Dis Model Mech

HBS1L deficiency causes retinal dystrophy in a child and a mouse model associated with defective development of photoreceptor cells

Authors - Shiyu Luo
View Article