验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse lung tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
18282-1-AP targets CCDC85B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | CCDC85B fusion protein Ag13129 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | coiled-coil domain containing 85B |
| Synonyms | Hepatitis delta antigen-interacting protein A, DIPA, Delta-interacting protein A, Delta interacting protein A, Coiled-coil domain-containing protein 85B |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 24-28 kDa |
| GenBank Accession Number | BC008796 |
| Gene Symbol | CCDC85B |
| Gene ID (NCBI) | 11007 |
| RRID | AB_2878527 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15834 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Coiled-coil domain-containing protein 85B (CCDC85B), also named as DIPA, is 202 amino acid protein, which belongs to the CCDC85 family. CCDC85B localizes in the nucleus and cytoplasm. CCDC85B is widely expressed in many tissues and functions as a transcriptional repressor. CCDC85B may inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. CCDC85B may play a role in adipocyte differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC85B antibody 18282-1-AP | Download protocol |
| IHC protocol for CCDC85B antibody 18282-1-AP | Download protocol |
| IF protocol for CCDC85B antibody 18282-1-AP | Download protocol |
| IP protocol for CCDC85B antibody 18282-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Prolif An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms | ||
