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验证数据展示

  • WB analysis of mouse stomach using 14947-1-AP

    WB analysis of mouse stomach using 14947-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 14947-1-AP

    IF Staining of A549 using 14947-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Cyclon/CCDC86 antibody (14947-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Cyclon/CCDC86 Polyclonal antibody

Cyclon/CCDC86 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14947-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

CYCLON, CCDC86, Cytokine-induced protein with coiled-coil domain, Coiled-coil domain-containing protein 86

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse stomach tissue, HepG2 cells, human heart tissue, human liver tissue, human stomach tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14947-1-AP targets Cyclon/CCDC86 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Cyclon/CCDC86 fusion protein Ag6754 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 86
别名 CYCLON, CCDC86, Cytokine-induced protein with coiled-coil domain, Coiled-coil domain-containing protein 86
计算分子量 40 kDa
观测分子量 65 kDa
GenBank蛋白编号BC001378
基因名称 CCDC86
Gene ID (NCBI) 79080
RRIDAB_2072318
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6F5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC86 (Coiled-coil domain-containing protein 86), also known as Cyclon (cytokine-induced protein with coiled-coil domain) ) is known for its expression in leukocytes in mice, where it regulates the immune response. Cyclon/CCDC86 regulates the apoptosis of T-cells upon activation, a process known as an activationinduced cellular death (AICD), by regulating the expression of the death receptor Fas on T-cells (PMID: 23439384). CCDC86 depletion led to a novel phenotype in which Ki-67 and nucleolin could still accumulate at the chromosome periphery but also formed abnormal cytoplasmic NDF-like foci (PMID: 36695333). The detected molecular weights (MWs) of Cyclon on SDS-PAGE (human Cyclon: 65 kDa; mouse Cyclon: 75 kDa) were higher than calculated MWs (human Cyclon: 40.2 kDa; mouse Cyclon: 46.5 kDa), suggesting possible modifications of Cyclon (PMID: 17300783).

实验方案

Product Specific Protocols
WB protocol for Cyclon/CCDC86 antibody 14947-1-APDownload protocol
IF protocol for Cyclon/CCDC86 antibody 14947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols