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CCDC93 Polyclonal antibody

CCDC93 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 20861-1-AP

Print datasheet

Synonyms

CCDC93



经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, human testis tissue, mouse colon tissue, Jurkat cells, human testis tissues
Positive IP detected inL02 cells
Positive IHC detected inhuman colon tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20861-1-AP is CCDC93 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CCDC93 fusion protein Ag14855 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 93
Synonyms CCDC93
Calculated Molecular Weight 631 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC028609
Gene Symbol CCDC93
Gene ID (NCBI) 54520
RRIDAB_10696446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ567U6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC93 antibody 20861-1-APDownload protocol
IHC protocol for CCDC93 antibody 20861-1-APDownload protocol
IP protocol for CCDC93 antibody 20861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.

Authors - Kerrie E McNally
mouseWB

Nat Commun

CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.

Authors - Paulina Bartuzi
humanWB

J Cell Biol

Endosomal sorting of Notch receptors through COMMD9-dependent pathways modulates Notch signaling.

Authors - Haiying Li
humanWB,IF

Elife

Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.

Authors - Sai Srinivas Panapakkam Giridharan
humanWB

Mol Metab

Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.

Authors - Liu Yang
humanCoIP

Mol Cancer Res

COMMD3-mediated endosomal trafficking of HER2 inhibits the progression of ovarian carcinoma

Authors - Shiqing Wang