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验证数据展示

  • WB analysis of mouse brain using 19431-1-AP

    WB analysis of mouse brain using 19431-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19431-1-AP (CCDC94 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 19431-1-AP

    IHC staining of human placenta using 19431-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19431-1-AP (CCDC94 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 19431-1-AP

    IHC staining of human placenta using 19431-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19431-1-AP (CCDC94 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of HeLa using 19431-1-AP

    WB analysis of HeLa using 19431-1-AP

    WB result of CCDC94 antibody (19431-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC94 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

CCDC94 Polyclonal antibody

CCDC94 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19431-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CCDC94

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19431-1-AP targets CCDC94 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CCDC94 fusion protein Ag13764 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 94
别名 CCDC94
计算分子量 323 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC000561
基因名称 CCDC94
Gene ID (NCBI) 55702
RRIDAB_10641994
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BW85
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

实验方案

Product Specific Protocols
WB protocol for CCDC94 antibody 19431-1-APDownload protocol
IHC protocol for CCDC94 antibody 19431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols