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验证数据展示

  • WB analysis using 68324-1-Ig

    WB analysis using 68324-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68324-1-Ig (CCDC94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HeLa using 68324-1-Ig

    WB analysis of HeLa using 68324-1-Ig

    WB result of CCDC94 antibody (68324-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC94 transfected HeLa cells.

  • IF Staining of A431 using 68324-1-Ig

    IF Staining of A431 using 68324-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC94 antibody (68324-1-Ig, Clone: 1D1G9 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

CCDC94 Monoclonal antibody

CCDC94 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68324-1-Ig

产品说明书

CloneNo. 1D1G9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

YJU2, Coiled-coil domain-containing protein 94, 1D1G9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68324-1-Ig targets CCDC94 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CCDC94 fusion protein Ag13772 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 coiled-coil domain containing 94
别名 YJU2, Coiled-coil domain-containing protein 94, 1D1G9
计算分子量 323 aa, 37 kDa
观测分子量40 kDa
GenBank蛋白编号BC000561
基因名称 CCDC94
Gene ID (NCBI) 55702
RRIDAB_2935396
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BW85
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

实验方案

Product Specific Protocols
WB protocol for CCDC94 antibody 68324-1-IgDownload protocol
IF protocol for CCDC94 antibody 68324-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols