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  • Featured Product
  • KD/KO Validated

CCDC99 Polyclonal antibody

CCDC99 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24689-1-AP

Print datasheet

Synonyms

CCDC99, hSpindly, MU-RMS-40.4A, Protein Spindly, SPDL1, spdly

验证数据展示

  • WB analysis using 24689-1-AP

    WB analysis using 24689-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24689-1-AP (CCDC99 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24689-1-AP

    WB analysis of HeLa using 24689-1-AP

    WB result of CCDC99 antibody (24689-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC99 transfected HeLa cells.

  • IHC staining of human liver cancer using 24689-1-AP

    IHC staining of human liver cancer using 24689-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 24689-1-AP

    IHC staining of human liver cancer using 24689-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293 using 24689-1-AP

    IP experiment of HEK-293 using 24689-1-AP

    IP result of anti-CCDC99 (IP:24689-1-AP, 4ug; Detection:24689-1-AP 1:2000) with HEK-293 cells lysate 1400 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HEK293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24689-1-AP targets CCDC99 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen CCDC99 fusion protein Ag20430 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 99
Synonyms CCDC99, hSpindly, MU-RMS-40.4A, Protein Spindly, SPDL1, spdly
Calculated Molecular Weight 605 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC012568
Gene Symbol CCDC99
Gene ID (NCBI) 54908
RRIDAB_2879673
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EA4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC99 antibody 24689-1-APDownload protocol
IHC protocol for CCDC99 antibody 24689-1-APDownload protocol
IP protocol for CCDC99 antibody 24689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

Cells

Identification of Ku70 Domain-Specific Interactors Using BioID2.

Authors - Sanna Abbasi
View Article
humanWB

PLoS One

Inhibiting the expression of spindle appendix cooled coil protein 1 can suppress tumor cell growth and metastasis and is associated with cancer immune cells in esophageal squamous cell carcinoma

Authors - Tao Liu
  • KD Validated
View Article