验证数据展示
经过测试的应用
| Positive WB detected in | SGC-7901 cells, mouse stomach tissue, rat stomach tissue, mouse pancreas tissue, rat pancreas tissue, mouse bladder tissue, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85205-1-RR targets CCKAR-specific in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cholecystokinin A receptor |
| 别名 | CCKAR, CCK1-R, CCK1 R, CCK AR, CCK A receptor |
| 计算分子量 | 48 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | NM_000730 |
| 基因名称 | CCKAR |
| Gene ID (NCBI) | 886 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P32238 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cholecystokinin (CCK) receptor, including CCKAR and CCKBR, is a receptor for cholecystokinin, mainly regulating pancreatic growth and enzyme secretion, and smooth muscle contraction of the gallbladder and stomach (PMID: 34556863). CCKAR (Cholecystokinin receptor type A) is primarily expressed in the alimentary tract, while CCKBR is mainly found in the brain and the stomach (PMID: 16816139). CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer (PMID: 36733361). The calculated MW of CCKAR is 48 kDa, but the observed MW is around 75 kDa, which may be with glycosylation modification.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCKAR-specific antibody 85205-1-RR | Download protocol |
| IP protocol for CCKAR-specific antibody 85205-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |