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Eotaxin Polyclonal antibody

Eotaxin Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 11786-1-AP

Print datasheet

Synonyms

C C motif chemokine 11, CCL11, Eosinophil chemotactic protein, Eotaxin, SCYA11, Small inducible cytokine A11

验证数据展示

  • IHC staining of human lung cancer using 11786-1-AP

    IHC staining of human lung cancer using 11786-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11786-1-AP (Eotaxin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 11786-1-AP

    IHC staining of human lung cancer using 11786-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11786-1-AP (Eotaxin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman lung cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

11786-1-AP targets Eotaxin in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivitymouse, rat
Immunogen Eotaxin fusion protein Ag2398 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-C motif) ligand 11
Synonyms C C motif chemokine 11, CCL11, Eosinophil chemotactic protein, Eotaxin, SCYA11, Small inducible cytokine A11
Calculated Molecular Weight 97 aa, 11 kDa
GenBank Accession NumberBC017850
Gene Symbol CCL11/Eotaxin
Gene ID (NCBI) 6356
RRIDAB_10860099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51671
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCL11 also named Eotaxin-1, was initially discovered as an eosinophil-specific chemoattractant. CCL11 plays a central role in both allergic and non-allergic inflammatory reactions by recruiting immune cells such as eosinophils,basophils and Th2 lymphocytes. CCL11 binds to the chemokine receptors CCR2, CCR3 and CCR5, with highest affinity to CCR3. High levels of CCL11 have been described in several chronic inflammatory diseases, such as allergic rhinitis, atopic dermatitis, asthma, gastrointestinal disease and rheumatoid arthritis.

实验方案

Product Specific Protocols
IHC protocol for Eotaxin antibody 11786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Am J Physiol Heart Circ Physiol

Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.

Authors - Li Lin
View Article
ratIHC

Am J Rhinol Allergy

Effects of Ursolic Acid on the Expression of Th1-Th2-related Cytokines in a Rat Model of Allergic Rhinitis After PM2.5 Exposure.

Authors - Na Sun
View Article