验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue, human heart tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 4 publications below |
| IF | See 2 publications below |
产品信息
22303-1-AP targets CCL18/MIP-4 in IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated) |
| 别名 | CCL18, MIP 4, MIP-4, CCL 18, C-C motif chemokine 18 |
| 计算分子量 | 10 kDa |
| GenBank蛋白编号 | NM_002988 |
| 基因名称 | CCL18/MIP-4 |
| Gene ID (NCBI) | 6362 |
| RRID | AB_2879066 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55774 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCL18 is a small cytokine belonging to the CC chemokine family. The effector functions that CCL18 exerts on the immune system is mainly on the adaptive immune system, while being produced and secreted mainly by innate immune system. Although the functions of CCL18 have been well studied in vitro, physiological effects of CCL18 in vivo have been difficult to characterize because there is no murine analog of CCL18. Only recently has the receptor for CCL18 been identified, and further characterization of CCL18 interactions with its receptor will lead to better understanding of the physiological role of CCL18 in humans.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCL18/MIP-4 antibody 22303-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Biomater Sci Eng Matrisome Provides a Supportive Microenvironment for Skin Functions of Diverse Species. | ||
Front Immunol Novel macrophage-related gene prognostic index for glioblastoma associated with M2 macrophages and T cell dysfunction | ||
J Cell Physiol Surgical trauma-induced CCL18 promotes recruitment of regulatory T cells and colon cancer progression. | ||
Nat Commun Single cell transcriptomic analyses implicate an immunosuppressive tumor microenvironment in pancreatic cancer liver metastasis | ||
Med Multimodal single-cell analyses reveal mechanisms of perianal fistula in diverse patients with Crohn's disease | ||
J Allergy Clin Immunol ALOX15+ M2 macrophages contribute to epithelial remodeling in eosinophilic chronic rhinosinusitis with nasal polyps |
