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CCL24/Eotaxin 2 Polyclonal antibody

CCL24/Eotaxin 2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 22306-1-AP

Print datasheet

Synonyms

C C motif chemokine 24, CCL24, CK beta 6, Ckb 6, Eotaxin 2, MPIF 2, MPIF2, SCYA24, Small inducible cytokine A24

验证数据展示

  • WB analysis of mouse thymus using 22306-1-AP

    WB analysis of mouse thymus using 22306-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22306-1-AP

    IHC staining of human breast cancer using 22306-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 22306-1-AP

    IHC staining of human breast cancer using 22306-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22306-1-AP (CCL24/Eotaxin 2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse thymus tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22306-1-AP targets CCL24/Eotaxin 2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-C motif) ligand 24
Synonyms C C motif chemokine 24, CCL24, CK beta 6, Ckb 6, Eotaxin 2, MPIF 2, MPIF2, SCYA24, Small inducible cytokine A24
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberNM_002991
Gene Symbol CCL24/Eotaxin 2
Gene ID (NCBI) 6369
RRIDAB_2879068
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO00175
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chemokine CCL24 is the second member of eotaxins, a group of eosinophils' selectively chemoattractants. CCL24 is constitutively expressed in fibroblasts, epithelial cells, macrophages. CCL24 has only one receptor CCR3, which mainly presents on eosinophils and was also found on basophils, monocytes, Th2 lymphocytes, epithelial cells and airway smooth muscles. CCL24 displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes.

实验方案

Product Specific Protocols
WB protocol for CCL24/Eotaxin 2 antibody 22306-1-APDownload protocol
IHC protocol for CCL24/Eotaxin 2 antibody 22306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Dis Markers

CCL24 Protects Renal Function by Controlling Inflammation in Podocytes.

Authors - Youdi Wang
View Article
human,mouseIHC,IF

Cell Death Dis

Mechanical stiffness promotes skin fibrosis via Piezo1-Wnt2/Wnt11-CCL24 positive feedback loop

Authors - Jiahao He
View Article