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CCL28 Polyclonal antibody, PBS Only

CCL28 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18214-1-PBS

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Synonyms

验证数据展示

  • IHC staining of human colon cancer using 18214-1-AP (same clone as 18214-1-PBS)

    IHC staining of human colon cancer using 18214-1-AP (same clone as 18214-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18214-1-AP (CCL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

  • WB analysis of human spleen using 18214-1-AP (same clone as 18214-1-PBS)

    WB analysis of human spleen using 18214-1-AP (same clone as 18214-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

产品信息

18214-1-PBS targets CCL28 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CCL28 fusion protein Ag12920 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-C motif) ligand 28
Synonyms
Calculated Molecular Weight 127 aa, 14 kDa
Observed Molecular Weight 8 kDa-9 kDa
GenBank Accession NumberBC069532
Gene Symbol CCL28
Gene ID (NCBI) 56477
RRIDAB_2262251
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRJ3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCL28, also named as SCYA28, CCK1 and MEC, belongs to the intercrine beta (chemokine CC) family. It has chemotactic activity for resting CD4, CD8 T-cells and eosinophils. CCL28 binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. CCL28 is expressed in a variety of human and mouse tissues, and it appears to be predominantly produced by epithelial cells. It is produced by epithelial cells of these tissues suggesting that this chemokine can play an important role by linking homing mechanisms between the gut, nasal mucosa and mammary gland (MG). In WB test, CCL28 is 14kd and 8-9kd(a putatively to a degradation fragment).