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验证数据展示

  • WB analysis using 12859-1-AP

    WB analysis using 12859-1-AP

    PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 12859-1-AP (CCL3L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CCL3L1 Polyclonal antibody

CCL3L1 Polyclonal Antibody for WB, ELISA
Cat No. 12859-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

464.2, C C motif chemokine 3 like 1, CCL3L1, CCL3L3, D17S1718, G0S19 2, LD78, LD78 beta(1 70), LD78BETA, MIP1AP, PAT 464.2, SCYA3L, SCYA3L1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12859-1-AP targets CCL3L1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 CCL3L1 fusion protein Ag3891 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-C motif) ligand 3-like 1
别名 464.2, C C motif chemokine 3 like 1, CCL3L1, CCL3L3, D17S1718, G0S19 2, LD78, LD78 beta(1 70), LD78BETA, MIP1AP, PAT 464.2, SCYA3L, SCYA3L1
计算分子量 10 kDa
观测分子量10 kDa
GenBank蛋白编号BC027888
基因名称 CCL3L1
Gene ID (NCBI) 6349
RRIDAB_3085401
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16619
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCL3L1 is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this gene binds to several chemokine receptors, including chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. The copy number of this gene varies among individuals, where most individuals have one to six copies, and a minority of individuals have zero or more than six copies. There are conflicting reports about copy number variation of this gene and its correlation to disease susceptibility.

实验方案

Product Specific Protocols
WB protocol for CCL3L1 antibody 12859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols