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验证数据展示

  • WB analysis of MCF-7 using 26270-1-AP

    WB analysis of MCF-7 using 26270-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26270-1-AP (CCM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 26270-1-AP

    IHC staining of human liver cancer using 26270-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26270-1-AP (CCM2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 26270-1-AP

    IHC staining of human liver cancer using 26270-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26270-1-AP (CCM2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 26270-1-AP

    IF Staining of MCF-7 using 26270-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 26270-1-AP (CCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

CCM2 Polyclonal antibody

CCM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26270-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

C7orf22, CCM2, Malcavernin, OSM, PP10187

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26270-1-AP targets CCM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 CCM2 fusion protein Ag24233 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cerebral cavernous malformation 2
别名 C7orf22, CCM2, Malcavernin, OSM, PP10187
计算分子量 49 kDa
观测分子量 52 kDa
GenBank蛋白编号BC004903
基因名称 CCM2
Gene ID (NCBI) 83605
RRIDAB_2880454
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BSQ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCM2 antibody 26270-1-APDownload protocol
IHC protocol for CCM2 antibody 26270-1-APDownload protocol
IF protocol for CCM2 antibody 26270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Endocrinology

Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice

Authors - Abraham Behrmann
  • KD Validated
View Article
humanWB

Biochem J

CCM2 and PAK4 act downstream of atrial natriuretic peptide signaling to promote cell spreading.

Authors - Koichi Miura
  • KD Validated
View Article