• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CCM2 Polyclonal antibody

CCM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26270-1-AP

Print datasheet

Synonyms

C7orf22, CCM2, Malcavernin, OSM, PP10187

验证数据展示

  • WB analysis of MCF-7 using 26270-1-AP

    WB analysis of MCF-7 using 26270-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26270-1-AP (CCM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 26270-1-AP

    IHC staining of human liver cancer using 26270-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26270-1-AP (CCM2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 26270-1-AP

    IHC staining of human liver cancer using 26270-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26270-1-AP (CCM2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 26270-1-AP

    IF Staining of MCF-7 using 26270-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 26270-1-AP (CCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26270-1-AP is CCM2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CCM2 fusion protein Ag24233 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cerebral cavernous malformation 2
Synonyms C7orf22, CCM2, Malcavernin, OSM, PP10187
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC004903
Gene Symbol CCM2
Gene ID (NCBI) 83605
RRIDAB_2880454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSQ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCM2 antibody 26270-1-APDownload protocol
IHC protocol for CCM2 antibody 26270-1-APDownload protocol
IF protocol for CCM2 antibody 26270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem J

CCM2 and PAK4 act downstream of atrial natriuretic peptide signaling to promote cell spreading.

Authors - Koichi Miura
  • KD Validated
View Article