验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CCNDBP1 Ready-To-Use IHC Kit. CCNDBP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12363-1-AP targets CCNDBP1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | CCNDBP1 fusion protein Ag3032 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin D-type binding-protein 1 |
| 别名 | HHM, Grap2 and cyclin-D-interacting protein, GCIP, DIP1, Cyclin-D1-binding protein 1 |
| 计算分子量 | 360 aa, 40 kDa |
| GenBank蛋白编号 | BC009689 |
| 基因名称 | CCNDBP1 |
| Gene ID (NCBI) | 23582 |
| RRID | AB_2877849 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95273 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCNDBP1, also named as DIP1, GCIP and HHM, is a helix-loop-helix protein which suppresses tumorigenesis. CCNDBP1 specifically interacts with one of the class III HDAC proteins, SirT6, which is important for maintaining genome stability. CCNDBP1 may negatively regulate cell cycle progression.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCNDBP1 antibody 12363-1-AP | Download protocol |
| IHC protocol for CCNDBP1 antibody 12363-1-AP | Download protocol |
| IF protocol for CCNDBP1 antibody 12363-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ. | ||
Front Oncol CCNDBP1, a Prognostic Marker Regulated by DNA Methylation, Inhibits Aggressive Behavior in Dedifferentiated Liposarcoma via Repressing Epithelial Mesenchymal Transition | ||
J Cell Mol Med LncRNA ABHD11-AS1 promotes the development of endometrial carcinoma by targeting cyclin D1. | ||
Oncol Rep Expression and prognostic significance of TRPV6 in the development and progression of pancreatic cancer. | ||
Am J Transl Res Lithium chloride inhibits cell survival, overcomes drug resistance, and triggers apoptosis in multiple myeloma via activation of the Wnt/β-catenin pathway. | ||
