Cyclin E2 Polyclonal antibody

Cyclin E2 Polyclonal Antibody for FC, IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

FC, IF, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11935-1-AP

Print datasheet

Synonyms

CCNE2, CYCE2, Cyclin E, cyclin E2, G1/S specific cyclin E2



经过测试的应用

Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11935-1-AP targets Cyclin E2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF, IHC, IP, ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen Cyclin E2 fusion protein Ag2532 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin E2
Synonyms CCNE2, CYCE2, Cyclin E, cyclin E2, G1/S specific cyclin E2
Calculated Molecular Weight 374 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC020729
Gene Symbol CCNE2
Gene ID (NCBI) 9134
RRIDAB_2228593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO96020
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.

实验方案

Product Specific Protocols
IHC protocol for Cyclin E2 antibody 11935-1-APDownload protocol
IF protocol for Cyclin E2 antibody 11935-1-APDownload protocol
IP protocol for Cyclin E2 antibody 11935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cancer

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

Authors - Xi Jin
humanWB

Elife

PMID: 30132758

Authors - Tonya Kueck
humanIHC

Mol Ther Nucleic Acids

miRNA in food simultaneously controls animal viral disease and human tumorigenesis.

Authors - Yalei Cui
humanWB

Mol Ther Nucleic Acids

lncRNA STEAP3-AS1 Modulates Cell Cycle Progression via Affecting CDKN1C Expression through STEAP3 in Colon Cancer.

Authors - Heya Na
humanWB

Oncotarget

Upregulation of E2F8 promotes cell proliferation and tumorigenicity in breast cancer by modulating G1/S phase transition.

Authors - Liping Ye
humanWB, IHC

Cancers (Basel)

A Patient-Derived Xenograft Model of Dedifferentiated Endometrial Carcinoma: A Proof-of-Concept Study for the Identification of New Molecularly Informed Treatment Approaches.

Authors - Chiao-Yun Lin