Cyclin E2 Polyclonal antibody

Cyclin E2 Polyclonal Antibody for WB, ELISA
Cat No. 30768-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

CCNE2, G1/S-specific cyclin-E2, G1/S specific cyclin E2, Cyclin E, CYCE2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30768-1-AP targets Cyclin E2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Cyclin E2 fusion protein Ag26029 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin E2
别名 CCNE2, G1/S-specific cyclin-E2, G1/S specific cyclin E2, Cyclin E, CYCE2
计算分子量 374 aa, 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC020729
基因名称 CCNE2
Gene ID (NCBI) 9134
RRIDAB_3086415
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO96020
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.

实验方案

Product Specific Protocols
WB protocol for Cyclin E2 antibody 30768-1-APDownload protocol
Standard Protocols
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