• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 16357-1-AP

    WB analysis using 16357-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16357-1-AP (cyclin I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 16357-1-AP

    IHC staining of human heart using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 16357-1-AP

    IHC staining of human heart using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

cyclin I Polyclonal antibody

cyclin I Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16357-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CCNI, CYC1, cyclin I, CYI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman heart tissue, human brain tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16357-1-AP targets cyclin I in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 cyclin I fusion protein Ag0493 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin I
别名 CCNI, CYC1, cyclin I, CYI
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC004975
基因名称 CCNI
Gene ID (NCBI) 10983
RRIDAB_2275606
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14094
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for cyclin I antibody 16357-1-APDownload protocol
IHC protocol for cyclin I antibody 16357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols