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CCNJL Polyclonal antibody

CCNJL Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26743-1-AP

Print datasheet

Synonyms

Cyclin-J-like protein, Cyclin J like protein, cyclin J like

验证数据展示

  • WB analysis using 26743-1-AP

    WB analysis using 26743-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26743-1-AP (CCNJL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26743-1-AP

    IF Staining of HepG2 using 26743-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CCNJL antibody (26743-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26743-1-AP targets CCNJL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CCNJL fusion protein Ag25048 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin J-like
Synonyms Cyclin-J-like protein, Cyclin J like protein, cyclin J like
Calculated Molecular Weight 435 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC013353
Gene Symbol CCNJL
Gene ID (NCBI) 79616
RRIDAB_3085900
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IV13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCNJL antibody 26743-1-APDownload protocol
IF protocol for CCNJL antibody 26743-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols