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CCNK Polyclonal antibody

CCNK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26933-1-AP

Print datasheet

Synonyms

CCNK, CPR4, cyclin K

验证数据展示

  • WB analysis using 26933-1-AP

    WB analysis using 26933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26933-1-AP (CCNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy CCNK Ready-To-Use IHC Kit. CCNK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26933-1-AP targets CCNK in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CCNK fusion protein Ag25556 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin K
Synonyms CCNK, CPR4, cyclin K
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC015935
Gene Symbol CCNK
Gene ID (NCBI) 8812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75909
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-K (CCNK) is also named as CPR4 and belongs to the cyclin family. CCNK is a regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases (PMID:10574912). It plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A) (PMID:9632813). The Cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes (PMID:22012619).

实验方案

Product Specific Protocols
WB protocol for CCNK antibody 26933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols